Figure 5. Avicin D induced cell killing is GR-independent.

(A) Western blot comparing the GR expression in wild type HEK293T cells transfected with the mock DNA and plasmid containing the wild type GRα. (B) HEK293T cells transfected with mock DNA or wild type GRα were treated with different concentrations of avicin D for 72 hrs. Cell viability was measured using the MTT assay as described in the methods. (C) Jurkat cells were treated with different concentrations of avicin D or Dex for 72 hrs. At the end of 72 hrs, cell viability was evaluated using the MTT assay, as described in the methods.