Figure 7. Role of different GR domains in avicin D-mediated inhibition of NF-κB activity.

HEK 293T cells were transiently transfected with mock DNA, or plasmids carrying either wild type GRα or deletion variants of GR. After transfection, cells were pre-treated with Avicin D (1 µM) for 2 hrs, followed by a 4 hrs treatment with TNF (1 nM). Luciferase activity was measured in the cell lysates, and normalized for TK renilla luminescence as described in the methods. Luciferase activity has been expressed as % change over the untreated control, which in turn is taken as 100%.