Table 2. Summary of DAF-16::GFP Nuclear Localization Data.
N | Foci positive Animals | % Positive | Mean # foci/animal | p-value | |
EV | 18 | 1 | 5.60% | 4.2 | NA |
daf-2(RNAi) | 35 | 9 | 25.70% | 5.7 | 0.035 |
FLA | 40 | 11 | 27.50% | 11.9 | 0.018 |
heat-shock | 10 | 10 | 100% | 115.9 | p>0.0001 |
Animals were scored for presence of nuclear puncta after treatment with DMSO, daf-2(RNAi), FLA or following a 2 hour heat-shock incubation at 37°C. A Student's T-Test was used to determine statistical significance.