Table 1. SPR Binding Constants for RhCMVIL-10 / human IL-10R1 Interactions.

	ka1 (1/Msec)	kd1 (1/sec)	ka2 (1/RUs)	kd2 (1/sec)	Rmax (RU)	Rm-obs* (RU)	KD1 (nM)	KD2 (nM)
RhCMVIL-10	1.18E+06	0.07671	0.001055	0.00155	24.3	20.9	65.1	1.3
RhCMVIL-10M1#	4,617	0.1985	2.32E-04	0.00187	24.3	0.8	43,000	410
RhCMVIL-10M2##	1.45E+04	0.2117	5.48E-04	0.00136	24.3	3.0	14,700	94.4

Rate constants were obtained from fitting the Sensorgrams in Figure 6 to a bivalent analyte model.

M = molar, RU = Response unit, KD1 = kd1/ka1, KD2 = kd2/ka1, Rmax is the calculated Rmax determined during fitting, Rm-obs is the maximum RU value observed in the sensorgrams at the highest concentration collected.

^#Binding parameters obtained by fixing Rmax and mass transport (tc = 1.546+E07) values to those obtained from global fitting of the RhCMVIL-10 data.

^##Binding parameters obtained by fixing Rmax to the value obtained from global fitting of the RhCMVIL-10 data.

*The theoretical Rmax for RhCMVIL-10 dimer binding to IL-10R1 is 29.5RU.