Table 4.
Assessment of assembly reliability on randomly selected contigs by using PCR with primers designed from BAC end sequences
| Contig ID | Number of primer pairs | Number of Clones | Number of Positive Clones | Contig assembly completely validated |
|---|---|---|---|---|
| 2042 | 15 | 65 | 65 | Yes |
| 113 | 12 | 85 | 85 | Yes |
| 2092 | 8 | 34 | 34 | Yes |
| 348 | 2 | 11 | 11 | Yes |
| 4368 | 4 | 11 | 11 | Yes |
| 1494 | 4 | 25 | 25 | Yes |
| 2220 | 4 | 14 | 14 | Yes |
| 4074 | 2 | 10 | 10 | Yes |
| 1258 | 2 | 13 | 13 | Yes |
| 6064 | 2 | 13 | 13 | Yes |
| 3806 | 4 | 17 | 17 | Yes |
| 4498 | 4 | 13 | 13 | Yes |
| 677 | 6 | 37 | 37 | Yes |
| 2995 | 4 | 24 | 24 | Yes |
| 3682 | 4 | 22 | 22 | Yes |
| 5929 | 2 | 7 | 7 | Yes |
| 3097 | 2 | 12 | 12 | Yes |
| 984 | 2 | 12 | 12 | Yes |