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. 2011 Nov 21;6(11):e27883. doi: 10.1371/journal.pone.0027883

Figure 3. DJ-1 oxidation induced by 6-OHDA.

Figure 3

(A) Cell samples obtained from the SH-SY5Y cells treated with 6-OHDA at indicated concentrations for 3 h were separated by 2D-PAGE and subsequently subjected to western blot analysis using antibodies against DJ-1 and oxidized DJ-1. (B) Cell samples obtained from the primary neuronal cells treated with 600 µM 6-OHDA for 3 h were separated by 2D-PAGE and subsequently subjected to western blot analysis using the anti-DJ-1 antibody. The ratio of oxidized DJ-1 is shown under each condition (n = 2).