Table 1.
Analysis of gene copy number in CBS7779 variants relative to strain H99.
| Strain | SMG1 (chr 4) | CNN00820 (chr 13) | CND00707 (chr 4) |
|---|---|---|---|
| H99 | 1 | 1 | 1 |
| JEC21 | 2.33 | 1.07 | 1.22 |
| CBS7779 B1 | 1.1 | 1.09 | ND |
| CBS7779 B2 | 1.08 | 0.95 | ND |
| CBS7779 B3 | 1.16 | 1.08 | ND |
| CBS7779 B4 | 1.1 | 1.03 | 1.08 |
| CBS7779 B5 | 1.24 | 0.99 | 1.28 |
| CBS7779 B6 | 1.16 | 1.01 | 1.37 |
| CBS7779 B7 | 1.27 | 0.68 | ND |
| CBS7779 B8 | 1.38 | 1.34 | ND |
| CBS7779 B9 | 1.43 | 1.01 | ND |
| CBS7779 B10 | 1.38 | 0.74 | ND |
| CBS7779 B11 | 1.26 | 1.15 | ND |
| CBS7779 B12 | 1.36 | 0.99 | ND |
| CBS7779 W1 | 1.3 | 1.84 | ND |
| CBS7779 W2 | 1.16 | 2.01 | ND |
| CBS7779 W3 | 1.1 | 1.97 | ND |
| CBS7779 W4 | 1.23 | 2.23 | 1.36 |
| CBS7779 W5 | 1.11 | 1.85 | 1.24 |
| CBS7779 W6 | 0.96 | 1.78 | 1.19 |
| CBS7779 W7 | 1.29 | 1.8 | ND |
| CBS7779 W8 | 1.13 | 2.66 | ND |
| CBS7779 W9 | 1.25 | 2.89 | ND |
| CBS7779 W10 | 1.15 | 2.01 | ND |
| CBS7779 W11 | 1.15 | 2.87 | ND |
| CBS7779 W12 | 1.13 | 3.1 | ND |
Quantitative PCR was used to examine copy number (2-ΔΔCt method).
ND, not determined