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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Oct 28;187(11):5495–5499. doi: 10.4049/jimmunol.1100521

Figure 4. γ-secretase inhibitor L-685458 decreases the ability of DCs to prime T cells in vivo.

Figure 4

CD11c bead purified DCs from C57Bl/6 mouse bone marrow were left untreated, treated with a low (15 μM per 106 DCs) or high dose (100 μM per 106 DCs) of γ-secretase inhibitor L-685458, followed by infection with BCG. Washed and viable DCs were injected i.p. to naive mice. 2 weeks later, spleen T cells were analyzed for IFN-γ producing CD4 T cells using flow cytometry(a), and Ag85B specific T cells using ELISPOT (b) (p values, t test; 2 experiments).