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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: J Neurosci Methods. 2011 Sep 24;203(1):141–145. doi: 10.1016/j.jneumeth.2011.09.007

Figure 1. Cell viability and neuroprotection assays using primary cultured cortical neurons in 96 well-plate assays.

Figure 1

(A) Representative images of primary cortical culture at 5 days in vitro. Cells differentiation was obvious by significant connections and dendrite formation. Calcein fluorescence indicates cell viability and activity of endogenous esterases. Scale bar: 10 µM. (B) Calcein fluorescence correlates with plating density of cells per well, at 5 days in vitro. Data is shown as mean ± s.e.m. (n=3). (C) tBHP treatment reduces cell viability, which was confirmed by MTT assay. Data is shown as mean ± s.e.m. (n=3). (D) Representative neuroprotection experiment utilizing 3-NP as insult and 1 mM Trolox™ as neuroprotectant. Trolox™ pre-treatment prevents 3-NP-induced loss in cell viability. Data is shown as mean ± s.d.