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. Author manuscript; available in PMC: 2012 Nov 25.
Published in final edited form as: Chembiochem. 2011 Oct 18;12(17):2564–2567. doi: 10.1002/cbic.201100545

Figure 3.

Figure 3

Molecular beacon probe for the analysis of human rs1490413 polymorphic site. A) Hybridization of MB probe to rs14 analyte. B) Fluorescent response of MB-rs14-G (20 nM) in the absence (dotted curve) or presence of complementary 500 nM rs14-G (dashed curve) or single base mismatched 500 nM rs14-A (solid curve) analytes. The fluorescent spectra were taken after 15 min of incubation at 22°C. All experiment have been done in optimal conditions for MB probe performance (concentrations and buffer) with exception that the temperature (22°C) was not optimized for SNP discrimination by MB probe.