Figure 2. Hepatic overexpression of cPLA₂α suppresses Fas-induced hepatocyte apoptosis and liver tissue damage.

The cPLA₂α Tg mice and their age/sex-matched wild type mice were administered intraperitoneally with saline or Jo2 ( 0.5 μg/g body weight ). The animals were sacrificed at 0 (a and b), 4 (c and d) and 6 (e and f) hours after injection and the liver tissues were harvested for histological evaluation. Formalin-fixed and paraffin-embedded sections (5 μm thick) were stained with hematoxylin and eosin (H&E) (A), and terminal deoxynucleotidy1 – transferase-mediated deoxyuridine triphosphate-digoxigenin nick-end labeling (TUNEL) (B) (200 × ). After Jo2 administration, the livers of the wild type mice exhibit more prominent hemorrhagic necrosis, hepatocyte apoptosis and degeneration (c and e), when compared to the livers of cPLA₂α Tg mice (d and f). The number of TUNEL-positive hepatocytes in the wild type mice is significantly higher than in the cPLA₂α Tg mice (*p<0.01 compared to the corresponding cPLA₂α Tg mice, the data are expressed as mean ±SD from 6 mice) (C).