Western analysis of proteins from AMD and normal retina. Human Bruch’s membrane/RPE/choroid from the macular region of AMD and normal donor eyes was subjected to SDS-PAGE, electroblotted to poly(vinylidene difluoride), and probed with the rabbit polyclonal anti-CEP antibody. CEP-modified human serum albumin (CEP-HSA) was used as a positive control. The age and sex of the donor eyes are listed at the top of each lane. More immunoreactivity is evident in the AMD samples than in the normals. This research was originally published in PNAS. Crabb, J. W., Miyagi, M., Gu, X., Shadrach, K., West, K. A., Sakaguchi, H., Kamei, M., Hasan, A., Yan, L., Rayborn, M. E., Salomon, R. G. and Hollyfield, J. G. (2002) Drusen proteome analysis: an approach to the etiology of age-related macular degeneration. Proc Natl Acad Sci U S A 99, 14682–14687. © The National Academy of Sciences of the United States of America.