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Reconstructed ion chromatograms of LQQC*PF (C-[S=O]-PhIP) and C*PF (C-[S=O]-PhIP) sulfinamide adducts obtained from HONH-PhIP- and NO-PhIP-modified plasma, followed by HiTrap Blue affinity purification of SA. A) LQQC*PF (C-[S=O]-PhIP) obtained by trypsin/chymotrypsin digestion of SA, and B) C*PF (C-[S=O]-PhIP) obtained by pronase E, leucine aminopeptidase, and prolidase digestion.
