Figure 2. CaMKIV suppresses cell cycle arrest of lupus MCs.
(A) and (B) MCs were stained by propidium iodide (PI) and cell cycle analysis was performed by flow cytometry. 400,000 MCs derived from MRL/MPJ and MRL/lpr mice were treated with or without KN-93 for 48 hrs and stimulated with or without PDGF-BB for 24 hrs. Cumulative data of flow cytometry (percentage of cells in each cell cycle phases) are shown from (A) unstimulated and (B) PDGF-BB-stimulated MCs. Values are the mean ± SD of n=4–5 mice/group and performed in three independently replicates. (C) 400,000 MCs derived from MRL/MPJ, MRL/lpr and MRL/lpr.Camkiv−/− mice were stimulated with or without PDGF-BB for 24 hrs. Cumulative data of flow cytometry (percentage of cells in each cell cycle phases) are shown. Values indicate mean ± SD of n=4–5 mice/group performed in three independently replicates.