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RPIP-HPLC to follow reaction progression over time for O-sulfonation of N-cbz neomycin. Following addition of ClSO3H, reaction aliquots were analyzed at: (A) 5 min, (B) 15 min, (C) 20 min, (D) 50 min, (E) 275 min. Percent of reaction product that is persulfated product (nz7) is indicated next to the nz7 peak at 28 minutes.
