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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Exp Eye Res. 2011 Aug 16;93(5):649–657. doi: 10.1016/j.exer.2011.08.004

Figure 5. Effect of MUC1/A and MUC1/B overexpression in TNFα-induced NF-κB activation.

Figure 5

COS-7 cells were transfected with NF-κB luciferase reporter, pRL-tk and 500 ng plasmid DNA for EV, MUC1/A or MUC1/B as described in Experimental Procedures. 48 h after transfection, wells were left untreated (UT) or treated with 1 or 10 ng/ml TNFα and WCE were harvested 4 and 24 h after treatment. Luciferase and Renilla luciferase activities were determined. Values were calculated as described in Experimental Procedures and are the mean ± SEM of 3 experiments. *, p<0.05 compared to EV. **, p<0.001 compared to EV. #, p<0.05 compared to MUC1/A. ##, p<0.001 compared to MUC1/A.