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Effects of genistein and daidzein on nitric oxide (NO) production in tumor necrosis factor (TNF)-α-stimulated human umbilical vascular endothelial cells (HUVECs). Cells (4 × 105 cells) in 24-well plates were preincubated with and without the indicated concentrations of genistein or daidzein for 2 hr and then incubated with TNF-α (10 ng/mL) for 20 hr. Data represent the means ± SD of triplicate experiments. Values sharing the same superscript are not significantly different at P < 0.05.
