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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Biomaterials. 2011 Nov 8;33(4):1065–1078. doi: 10.1016/j.biomaterials.2011.10.046

Figure 4.

Figure 4

Quantitative histomorphometric analysis for bone loss and TRAP enzyme activity for osteoclast quantification. (a) Quantitative morphometric measurements of the degree of bone loss showing significant loss of bone for those co-cultures of calvarial bone with MCF-7 (60%), MDA-231 (70%) and PC3 (50%) compared to bone grown alone. LnCap co-cultures showed much less bone loss (15%) and 20 nM PGE2 about 20%. The two sample student's t test indicated that the difference in bone resorption in response to cancer cells and added PGE2 were different and statistically significant with p values as noted within the Figure. (b) Quantitative TRAP enzyme activity analysis. The two sample student's t test indicated that the difference in TRAP activity in response to cancer cells and added PGE2 were different and statistically significant with p values as noted within the Figure.