Expression of thrombopoietin (TPO) in liver (A) and VAT (B) and relative TPO levels in liver vs. VAT (C) were evaluated by real-time RT-PCR in lean WT (red), lean KO (yellow), DIO WT (green) and DIO KO (blue) mice. Plasma (D) and liver-associated (E) TPO levels (C) were evaluated by ELISA. Expression of IL-11 in liver (F) and VAT (G) was evaluated by real-time RT-PCR
Data are mean +/− SEM of 8-10 mice per group. Data in panels A, B, F and G are reported as gene expression relative to the WT lean group using the ΔΔct method. Data in panel C are reported as gene expression relative to the respective VAT value using the ΔΔct method.
^p<0.05 vs lean WT; *p<0.05 vs lean KO by ANOVA.