Table 1. Hematological parameters in lean and DIO WT and KO mice.
| Lean WT | Lean KO | DIO WT | DIO KO | |
|---|---|---|---|---|
| WBC (103/μl) | 7.8 +/− 0.4 | 6.9 +/− 0.4 | 10.0 +/− 1.0* | 7.7 +/− 0.6 |
| # NE (103/μl) | 1.6 +/− 0.1 | 1.7 +/− 0.1 | 2.6 +/− 0.6* | 1.8 +/− 0.1 |
| # LY (103/μl) | 5.7 +/− 0.3 | 4.7 +/− 0.2 | 6.6 +/− 0.7* | 5.5 +/− 0.5 |
| # MO (103/μl) | 0.4 +/− 0.04 | 0.3 +/− 0.03 | 0.5 +/− 0.07 | 0.4 +/− 0.05 |
| # EO (103/μl) | 0.07+/− 0.03 | 0.06 +/− 0.02 | 0.1 +/− 0.07 | 0.08 +/− 0.03 |
| # BA (103/μl) | 0.02 +/− 0.01 | 0.01 +/− 0.01 | 0.02 +/− 0.01 | 0.01 +/− 0.01 |
| %NE | 22.4 +/− 1.0 | 24.3 +/− 1.0 | 22.7 +/− 0.6 | 24.3 +/− 1.3 |
| % LY | 71.5 +/− 1.2 | 69.6 +/− 1.0 | 71.9 +/− 0.8 | 69.5 +/− 1.2 |
| %MO | 5.1 +/− 0.4 | 5.1 +/− 0.3 | 4.9 +/− 0.5 | 5.2 +/− 0.3 |
| %EO | 0.8+/− 0.3 | 0.8 +/− 0.2 | 0.8 +/− 0.4 | 0.9 +/− 0.2 |
| %BA | 0.2 +/− 0.1 | 0.1 +/− 0.1 | 0.2 +/− 0.1 | 0.2 +/− 0.1 |
| RBC (106/μl) | 9.4 +/− 0.3 | 9.1 +/− 0.1 | 9.8 +/− 0.2 | 9.8 +/− 0.5 |
| Hgb (g/dl) | 12.2 +/− 0.3 | 12.3 +/− 0.1 | 12.8 +/− 0.3 | 12.6 +/− 0.6 |
| Platelets (103/μl) | 938 +/− 36 | 847 +/− 31 | 873 +/− 23 | 690 +/− 37* |
| MPV (fl) | 4.6 +/− 0.1 | 4.4 +/− 0.1 | 4.5 +/− 0.1 | 4.6 +/− 0.1 |
| % ret. platelets | 2.7 +/− 0.5 | 2.5 +/− 0.1 | 2.2 +/− 0.2 | 2.6 +/− 0.2 |
| # MK/field | 27.6 +/− 2.7 | 31.4 +/− 2.7 | 33.6 +/− 2.4 | 36.4 +/− 2.8 |
White blood cell (WBC) counts, Neutrophil (NE), Lymphocyte (LY), Monocyte (MO), Eosinophil (EO) and Basophil (BA) absolute numbers and percentages, Red Blood cell (RBC) numbers, Hemoglobin (Hgb) levels, platelet number and Mean Platelet Volume(MPV) were evaluated using EDTA-anticoagulated peripheral blood obtained from lean and DIO WT and KO mice using a Hemavet 950FS. The percentage of reticulated platelets (ret platelets) was evaluated on EDTA-anticoagulated peripheral blood using the thiazole orange assay as described in the Methods section. The number of megakaryocytes (MK) in the bone marrow was evaluated on H&E sections as described in the Methods section.
Data are mean +/− SEM of 8-10 mice per group.
p<0.05 vs each other group by ANOVA.