Figure 9. Regulatory activity of G107S CD8⁺ and DN T cells in B6 mice and mixed chimeras.

(A) 10⁵ of the indicated flow cytometrically purified T cell subset or saline was transferred i.v. into B6 mice 1 d prior to EAE induction and disease course monitored. Data is pooled from 2 similar experiments. N=10 per group. Mean ± 1 s.e.m is plotted. (B) Engraftment of G107S or 1MOG9 T cell subsets in mixed chimeras. HPC transduced with the indicated TCR were admixed with wt B6 BM cells and transplanted into irradiated B6 recipients. The retrogenic T cells were identified in blood cells by co-expressed GFP. Mean + 1 s.d. is plotted. Representative of 2 experiments. (C) EAE was induced in mice in (B) and disease clinically monitored. N=7, G107S; N=6 other groups. Mean ± 1 s.e.m is plotted.