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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Oct 31;187(11):5834–5841. doi: 10.4049/jimmunol.1100922

FIGURE 3.

FIGURE 3

(A) In vitro treatment of cIELs from IL-10−/− mice with severe pathology overnight with recombinant 50 ng/ml rIL-10 resulted in the suppression of 6 of 11 miRNAs relative to cells cultured with PBS (* ≤ 0.05). (B) TargetScan (Release 5.1) analysis of the mouse Roquin (Rc3h1) 3′UTR revealed a potential target site for mmu-miR-223. Alignment of mmu-miR-223 to the conserved site is shown. (C) Intracellular expression of IL-17 and IFNγ in IL-10−/− cIELs. In vitro IL-10 treatment of cIELs from IL-10−/− mice resulted in significant reduction in (D) IL-17 mRNA expression and (E) IL-17A secretion as determined by ELISA. (F) cIELs from IL-10−/− mice have lower Roquin gene expression. (G) Culture of cIELs from IL-10−/− mice for 24 hours with 50 ng rIL-10 results in an increase in Roquin gene expression. (H-I) Transient transfection of EL4 cells with Roquin-specific siRNA oligonucleotides resulted in suppression of Roquin gene expression and enhanced IL-17 gene expression. Determination of statistical significance was calculated using Student’s t-test.