Figure 2.

HPLC chromatograms of a carbohydrate standard (A), water-soluble carbohydrates (B), and sedoheptulose reductase reaction products (C) of fully mature polyanthus leaves. Carbohydrates were extracted by the cryo-sap method and determined by HPLC-PAD. The sedoheptulose reductase assay was performed as described in Methods. The control in C was a boiled enzyme blank incubated for 30 min. 1, Raffinose; 2, Suc; 3, galactinol; 4, Glc; 5, sedoheptulose; 6, Fru; 7, myo-inositol; 8, volemitol; 9, perseitol.