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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Chem Res Toxicol. 2011 Sep 7;24(11):1891–1898. doi: 10.1021/tx200237a

Figure 2.

Figure 2

Targeting strategy to delete exon 5 of the ES1 gene. The top drawing shows the first 7 exons of the wild-type allele, as well as the location of the 5′ and 3′ homology arms in the gene targeting vector, the location of two probes (5′ probe and 3′ probe) outside the gene targeted region, and the location of one internal probe. Also shown are the restriction sites used for Southern blotting to distinguish wild-type, targeted, and deleted alleles. The middle drawing shows the targeted allele after recombination has inserted loxP sites and the Neo gene cassette. The bottom drawing shows the deleted allele produced by breeding to Cre recombinase-expressing mice, thus deleting exon 5 and the Neo gene cassette.