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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Immunol. 2011 Oct 31;187(11):5921–5930. doi: 10.4049/jimmunol.1100746

Fig. 3.

Fig. 3

Glyphosine specifically enhances the T cell response to insulin B:9–23 presented by I-Ag7. (A) Glyphosine enhanced stimulation of the T cell hybridoma 8-1.1α1 only when insulin B:9–23 peptide is present. Glyphosine concentrations are indicated and the reaction mixture contained B:9–23 peptide unless otherwise noted. The glyphosine concentration used in subsequent experiments was 500 nM. TT = tetanus toxin (negative control peptide). *p=0.011, **p=0.049 (unpaired Student’s t-test). (B) The glyphosine response in the presence of B:9–23 is blocked by a monoclonal antibody to I-Ag7 and unchanged with an isotype control antibody. *p<0.001. (C–D) Glyphosine does not alter TCR reactivity to a chromogranin peptide presented by I-Ag7 to the BDC 2.5 T cell hybridoma or to ovalbumin presented by I-Ad to the DO11.10 T cell hybridoma. Results are given as a stimulation index (SI) from triplicate cultures ± SEM. Data are representative of at least three independent experiments.