FIGURE 1.
Study protocols. A: Protocol 1: Mice were exposed to 10 intranasal (I.N.) HDM doses over 24 days. Rapamycin treatment by intraperitoneal injection (I.P.) was started simultaneously with HDM exposure and continued 6 days per week until one day after the last HDM exposure for a total of 20 injections. B: Protocol 2: Mice were sensitized to HDM by 3 I.P. injections before I.N. challenge with saline or HDM. Rapamycin treatment was started after the last I.P. injection and one day prior to I.N. HDM exposure.