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Sequence of Primers
| Cytokine family | Cytokine | 5'Primer | 3'Primer |
|---|---|---|---|
| Th1 | IFN-γ | 5'AAT GAA CGC TAC ACA CTG CAT 3' | 5' TGG CAG TAA CAG CCA GAA ACA 3' |
| Th1 | TNF-α | 5' GGG CAG GTC TAC TTT AGA GTC ATT G 3' | 5'GGC TGG GTA GAG AAT GGA TGA A 3' |
| Th2 | IL-4 | 5'GCA GAG ACT CTT TCG GGC TTT 3' | 5'TCA TTC ATG GTG CAG CTT ATC G 3' |
| reference Gene | HPRT | 5' GAA AGA CTT GCT CGA GAT GTC A 3' | 5' AGC ACA CAG AGG GCC ACA A 3' |
