Figure 3.

Apoptosis in three different splenic lymphocyte populations from P. chabaudi AS infected mice treated with AG or LPS. The mice were infected and treated as described in Figure 2. At the indicated days, the mice were sacrificed and the cells were isolated from the spleen and stained for lymphocytic markers (B220⁺, CD4⁺ and CD8⁺) and for apoptosis before analysis by flow cytometry. The histograms represent the percentage of apoptosis in each cell subpopulation. Each bar represents the average number of cells from three independent mice ± standard deviation. * indicates statistical significance compared to control mice treated with PBS on the indicated days. CT: control; AG: aminoguanidine; LPS: lipopolysaccharide. Each result is representative of at least two different experiments.