Figure 4.

qRT-PCR of IFN-γ and IL-10 in three subpopulations of spleen cells on day 11 of P. chabaudi AS infection. Eleven days after the infection, CD4⁺, CD8⁺ or NK.1 (NK) positive cells were isolated from the total spleen cells from control (PBS) or P. chabaudi AS-infected mice (I) treated with or without aminoguanidine (AG). cDNA from each population was used to perform qRT-PCR using primers specific for IFN- γ, IL-10 or β-actin. All of the procedures were performed as described in the Material and Methods. The results are expressed as the average value ± the standard deviation of the mean of the relative signals for each cytokine normalised to β-actin. n = 6, * indicates statistically significant differences.