Figure 5.

qRT-PCR of TNF-α in three subpopulations of spleen cells at day 4 of P. chabaudi AS infection. Four days after the infection, CD4⁺, CD8⁺ or NK⁺ cells were isolated from total spleen cells as described in the Material and Methods section. The control animals were injected with PBS and the experimental groups were infected with P. chabaudi AS (I) and treated with or without aminoguanidine (AG). cDNA from each population was used to perform qPCR using primers specific for TNF-α or β-actin. All of the procedures were performed as described in the Materials and Methods section. The results are expressed as the average values ± the standard deviation of the mean of the relative TNF-α signal normalised to β-actin. n = 6.