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. Author manuscript; available in PMC: 2012 Nov 11.
Published in final edited form as: Chemistry. 2011 Sep 28;17(46):13052–13058. doi: 10.1002/chem.201101987

Figure 1.

Figure 1

Real-time detection of a single base substitution in stem-loop folded nucleic acids using an MB probe. a) Direct hybridization of an MB probe to the stem-loop folded analyte is inefficient, since two hairpin-folded oligonucleotides are in lower energy state than the duplex. b) A tricomponent probe for SNP analysis in folded nucleic acids. The quaternary complex is thermodynamically stabilized by the formation of a long duplex between strand f and the analyte. Triethylene glycol (TEG) linkers are shown as dashed lines. Asterisks represent SNP sites.