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Tricomponent probe can detect single base substitutions in folded analytes. a) Signal-to-background ratio for the probe f1/m1 in the presence of the true target T13, and the two mismatched targets T13-12A and T13-16C. b) Signal-to-background ratio for the probe f2/m2 in the presence of the true target and the mismatched T13-8A. The data are average values of three independent measurements. Error bars represent standard deviations from the average.
