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Chromatogram of a Driselase digest of the SDS-washed cell walls on DEAE-Sepharose. About 10 g of the SDS-washed cell walls was hydrolyzed with Driselase and the digest was chromatographed on a DEAE-Sepharose column (1.6 × 17 cm), that had been equilibrated with 20 mm Tris-HCl (pH 8.0). The pectic fragments were eluted with a 1-L gradient of 0 to 0.5 m NaCl in 20 mm Tris-HCl (pH 8.0).
