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. 2011 Oct 26;31(43):15407–15415. doi: 10.1523/JNEUROSCI.2745-11.2011

Figure 2.

Figure 2.

Temporal expression of BDNF mRNA and protein in the embryonic lung. A, Analysis of the temporal expression of BDNF in the developing lung between E11.5 and E15.5 by Western blot analysis. B, Relative levels of BDNF protein were quantified by normalizing to the GAPDH internal control; **p = 0.007, *p = 0.03. C, Temporal quantification of BDNF mRNA levels by quantitative RT-PCR using β-actin mRNA as internal control; *p = 0.04. D, In situ hybridization to detect BDNF mRNA expression in E12.5 and E13.5 lungs. Lung epithelium is outlined by dotted lines. The sense probe was used as a control for background signal; n.s., Not significant. Data represent mean and SEM of three independent experiments.