Figure 5.

Synergistic effect of ABA and NaCl in inducing endogenous RD29A expression at room temperature. Nine-day-old plants on MS agar plates were transferred to filter paper saturated with MS salt solution and sprayed with water (Control) or with 100 μm ABA (ABA), or were treated with 300 mm NaCl in the MS salt background and sprayed with water (NaCl) or 100 μm ABA (NaCl + ABA). The treatment lasted for 4 h under cool-white light at 22°C ± 2°C, and total RNA was extracted immediately after the treatment.