Figure 6.

Cold shock (4.5 h at 0°C) inhibits RD29A-LUC expression in response to other stress treatments. Experiments were done with plants on filter paper soaked with either MS salt solution (for control or ABA treatments) or 300 mm NaCl in MS solution (for NaCl or ABA plus NaCl treatments). For combined treatments of 4.5 h cold plus other stresses, plants in agar plates were first incubated at 0°C for 30 min before being transferred to filter paper for ABA and/or NaCl treatments for 4 h in the cold. Plants given the normal cold treatment (2 d) were first incubated at 0°C on agar plates for 44 h before being transferred to filter paper for ABA and/or NaCl treatments for 4 h in the cold. Luminescence images were taken immediately after the treatments. White bars, 22°C ± 2°C; hatched bars (not visible), 4.5 h of cold treatment; and black bars, 2 d of cold treatment. Error bars represent ±sd (n = 15).