Figure 7.

Brief cold shock impaired the induction of endogenous RD29A expression by ABA and NaCl. Nine-day-old plants on MS agar plates were sprayed with 100 μm ABA or transferred to filter paper saturated with 300 mm NaCl in the MS salt background. The treatments lasted for 4 h under light at 22°C ± 2°C. Plants were first incubated on ice in a cold room under light for 30 min and then sprayed with 100 μm ABA (ABA+4.5hCold) or transferred to filter paper saturated with 300 mm NaCl in the MS salt background (NaCl+4.5hCold). The treatments lasted for 4 h under light in the cold. Total RNA was extracted immediately after the end of the treatments.