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. 2012 Jan 15;16(2):113–127. doi: 10.1089/ars.2011.3964

FIG. 3.

FIG. 3.

HO-1 inhibition on differentiation of C2C12 cells. (A–C) Effect of HMOX1 inhibition with SnPP (5 days, 50 μmol/L) on expression of MyoD mRNA (A), myogenin mRNA (B), and MHC mRNA (C) in C2C12-Luc-GFP-HO1 cells cultured in GrM or DfM. (D–G) Effect of scrambled (sc) or HMOX1 siRNA on expression of HMOX1 mRNA (D), MyoD mRNA (E), myogenin mRNA (F), and MHC mRNA (G) in C2C12-Luc-GFP-HO1 cells cultured for 5 days in GrM or DfM. QRT-PCR. EF2 served as a constitutive gene. Each bar represents mean±SEM of 3 experiments. *p<0.05, **p<0.01 versus C2C12-Luc-GFP cells, #p<0.05, ###p<0.001 versus cells cultured in GrM. (H) Immunofluorescent staining of MHC protein (red) in C2C12-Luc-GFP cells and C2C12-Luc-GFP-HO1 cells transfected with sc or HMOX1 siRNA and cultured for 5 days in GrM or DfM (blue: staining of nuclei with DAPI). Representative result.