FIGURE 2.

Mitochondrial lncRNAs are regulated by nuclear-encoded proteins involved in mitochondrial RNA processing. (A) Strand-specific qRT–PCR showing the effects of mitochondrial RNA processing proteins on lncRNAs. The effects of ELAC2, MRPP1, MRPP3, PTCD1, and PTCD2 knockdown in HeLa cells were analyzed on mitochondrial transcripts by ss qRT–PCR. (B) RNA isolated from cells transfected with ELAC2, MRPP1, MRPP3, PTCD1, or PTCD2 siRNA and nontargeting (NT) siRNA was analyzed for mitochondrial noncoding and complementary coding RNA abundance by Northern blotting. (C) The 12S rRNA and the complementary noncoding region of 12S rRNA were probed as controls. The data are typical of results repeated on four separate RNA preparations. Each Northern blot was probed for all transcripts by stripping the blot and reprobing. The methylene blue-stained blot shows equal loading of RNA to each well.