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Duplexed antisense and sense RNAs accumulate in the RNase J–deficient leaves. Total RNA isolated from wild-type (WT) or RNase J–deficient material was treated with increasing amounts of mung bean nuclease (0, 0.15, 1.5, 15, or 150 units) for 15 min prior to gel blot analysis. Blots were hybridized with strand-specific rbcL (A) or atpB (B) RNA probes to detect transcripts from the indicated strands.
