Hydrogen bond interactions between some selected residues around the wobble base pair for the ASL (modified in blue, unmodified in green) bound to cytosine (A), adenine (B), uracil (C), and guanine (D). Direct interactions are shown in dark colors and the water-bridged are added on top with light colors. (R) Ribosomal residues, (m) mRNA, (t) tRNA.