FIGURE 5.

p53-induced gene expression. (A) HT29-tsp53 cells were incubated at the permissive temperature for the indicated time (between 0 and 24 h), and RNA was collected for qRT-PCR to monitor the induction of 10 separate p53-induced transcripts (CRYAB, TP53I3, BTG2, CDKN1A, DDB2, FAS, GDF15, MDM2, SERPINB5, and XPC). Expression of the indicated representative mRNA is expressed as fold-change compared with samples maintained at the restrictive temperature. (B) Data in A were normalized to the expression level determined following 6 h at the permissive temperature. (C) The mean response of two stable and eight unstable p53-induced transcripts, as normalized in B. (D) Data in A were normalized to the expression level determined following 24 h at the permissive temperature. (E) The mean response of two stable and eight unstable p53-induced transcripts, as normalized in D. Each value in A, B, and D represents the mean (±SEM) determined from a minimum of three independent experiments.