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. 2011 Dec 15;138(24):5369–5378. doi: 10.1242/dev.051888

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Fig. 2. — T-Cre; Fgf8^flox/^Δ^2,³ mice fail to form mesonephric tubules. (A,B) Hematoxylin and Eosin stained sections (×400) of normal (A) and T-Cre; Fgf8^flox/^Δ^2,³ (B) embryos at E10.5. Black arrows label the mesonephric tubules and black arrowheads indicate the WD. The mesonephric tubules were not formed in T-Cre; Fgf8^flox/^Δ^2,³ embryos. (C,D) Abbreviated whole-mount in situ hybridization staining (overnight, 4°C) for Pax2 (in order to label the WD but not the mesonephric tubules) reveals that the WD (black arrowheads) normally extends to the junction of the forelimb and trunk (red arrows) in E10.5 controls but not in mutants (D) due to an anterior truncation. (E,F) TUNEL staining shows the premature degeneration of the Wolffian duct (arrowheads) in E9.5 mutants. (G,H) Lengthy whole-mount in situ hybridization staining (4 days, 4°C) for Pax2 in E10.5 embryos shows the presence of only the caudal mesonephric tubules in T-Cre; Fgf8^flox/^Δ^2,³ embryos (black arrowhead, WD). Scale bars in A,B,E,F: 20 μm. ca, caudal mesonephric tubules; cr, cranial mesonephric tubules.