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. Author manuscript; available in PMC: 2011 Nov 22.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2010 Aug 2;1(2):266–286. doi: 10.1002/wrna.31

Figure 5. Schematic of Ago HITS-CLIP.

Figure 5

UV crosslinking of intact tissues creates covalent bonds between Ago-miRNA and, separately, Ago in contact with neighboring mRNA sequences (structure on left is adapted from 117). Alignment of the latter produces an Ago-mRNA footprint; analysis of this restricted sequence space allows prediction and validation of miRNA binding sites. (Figure adapted from 89).