Figure 1.

Mechanical wounding, electrical current, and heat treatments initiate the local and systemic accumulation of Pin2 mRNA. Wild-type (MM) and ABA-deficient (notabilis, flacca, and sitiens) mutant tomato plants were wounded or treated with electrical current or heat. Six hours after treatment, total RNA was isolated from the treated leaves (L) and from leaves located distal (S) to the treated ones. The autoradiogram shows the result of a gel-blot hybridization of total RNA (10 μg per slot) against radioactive Pin2 (Sanchez-Serrano et al., 1986).