Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid |
Relevant characteristic(s) | Source or reference |
|---|---|---|
| B. subtilis GRSW2-B1 | Butanol-tolerant bacterium | This study |
| B. subtilis 168 | A type-strain Bacillus subtilis. Source of promoter sequences: P43, P2L | Laboratory stock |
| E. coli DH5α | hsdR17 recA endA1 lacZΔM15. For plasmid construction and propagation purpose | Invitrogen, USA |
| Plasmids | ||
| pHY300PLK | A shuttle vector for E. coli and B. subtilis, carrying bla (Apr) and tetL (Tcr). Source of tetracycline promoter (PTet) | (Ishiwa and Shibahara 1985) |
| pQF50 | A broad-host range vector. Source of trpA terminators, a multiple cloning site (MCS) and lacZ | (Farinha and Kropinski 1990) |
| pUC4K | A vector carrying Apr, Kmr. Source of kanamycin promoter (PKm) | Laboratory stock |
| pNCMO2 | A vector carrying strong promoter P2 for Brevibacillus. Source of P2 promoter (P2N) | Takara Bio Inc., Japan |
| pDG148 | A shuttle vector for E. coli and B. subtilis, carrying bla (Apr) and kan (Kmr). Source of kanamycin resistant gene cassette and Spac promoter (PSpac) | Laboratory stock |
| pWH1520 | An expression vector for B. megaterium. Source of xylose promoter (PxylA) | Mo Bi Tec, Germany |
| pHZT | pHY300PLK (Tcr) carrying trpA, MCS, lacZ | This study |
| pHZK | pHY300PLK, carrying trpA, MCS, lacZ, and tetL (Tcr) was replaced with kan (Kmr) | This study |
| pHZT-P43 | pHZT carrying P43 | This study |
| pHZT-PK | pHZT carrying PKm | This study |
| pHZT-P2N | pHZT carrying P2N | This study |
| pHZT-P2L | pHZT carrying P2L | This study |
| pHZT-PT | pHZT carrying PTet | This study |
| pHZT-PS | pHZT carrying PSpac | This study |
| pHZT-PX | pHZT carrying PxylA | This study |
| pHZK-PX | pHZK carrying PxylA | This study |