Table 3.
Recovery factor percentage
| soybean seeds (*) | R4 (**) [cfu.seed-1] | R0 (**) [cfu.seed-1] | RFP 4 hours at 20°C (***) |
|---|---|---|---|
| 4870 sample 1 (AD) | 1.90 105 | 1.47 106 | 12.9 |
| 4870 sample 2 (AD) | 1.52 105 | 1.12 106 | 13.6 |
| 4870 sample 3 (AD) | 1.53 105 | 9.82 105 | 15.6 |
| 3810-pb sample 1 (AD) | 1.40 105 | 2.65 106 | 5.28 |
| 3810-pb sample 1 (PD) | 4.00 105 | 2.65 106 | 15.1 |
| 3810-pb sample 2 (AD) | 1.20 105 | 2.50 106 | 4.8 |
| 3810-pb sample 2 (PD) | 3.90 105 | 2.50 106 | 15.6 |
Bacterial direct count and RFP at 20°C after 4 hours of inoculation with B. japonicum strain 61A273 into two commercial soybean seeds
R4: cfu.seed-1 4 hours after inoculation; R0: cfu.seed-1 theoretically provided by inoculant; RFP: Recovery Factor (%).
(*) cv. 4870: 6,200 seeds.kg-1 and cv. 3810-pb: 6,000 seeds.kg-1
(**) average of three determinations
(***) bacterial counting in inoculant used for cv. 4870: sample 1 (3.04 109 cfu.ml-1); sample 2 (2.32.109 cfu.ml-1); sample 3 (2.03.109 cfu.ml-1).bacterial counting in inoculant used for cv. 3810-pb: sample 1 (5.3 109 cfu.ml-1); 3810-prebásica sample 2 (5.0 109 cfu.ml-1).
(AD) treated with a dose of 3 ml.kg-1 seed of the inoculant (agronomic dose)
(PD) treated with a dose of 3 ml.kg-1 seed of inoculant and 0.3 ml.kg-1of sucrose 80% solution