Table 3.
Production and distribution of inulinase in static batch cultures of K. marxianus DMKU 3-1042 in YP medium with 2% various carbon substrates
| Medium | Temperature (°C) | Total inulinase activity (U mg of cell dry wt-1)a | Inulinase activity (U mg of cell dry wt-1)a | |
|---|---|---|---|---|
| Supernatant (%) | Cell (%) | |||
| YPD | 30 | 600 | 310 (52) | 290 (48) |
| 45 | 1140 | 880 (77) | 260 (23) | |
| YPSuc | 30 | 650 | 310 (48) | 340 (52) |
| 45 | 1290 | 1000 (78) | 290 (22) | |
| YPInu | 30 | 1920 | 1000 (52) | 920 (48) |
| 45 | 1390 | 820 (59) | 570 (41) | |
| YPDSuc | 30 | 640 | 410 (64) | 230 (36) |
| 45 | 1570 | 1300(83) | 270 (17) | |
| YPDInu | 30 | 590 | 320 (54) | 270 (46) |
| 45 | 1230 | 960 (78) | 270 (22) | |
a Enzyme activities were measured with sucrose as substrate with samples taken at 6 h from static batch cultures as described in Materials and methods
Values are average from two independent experiments