Table 1.

Purification of 2,5-DKCMO via nickel-based affinity chromatography and imidazole removal

Step	V [mL]	Volumetric activitya [U/mL]	Activitya [U]	Protein amountb [mg/mL]	Specific activity [mU/mg]	Yield [%]	Factor
Crude extract	30	0.0021	0.063	14	0.15	100	1
Purified and desalted	7	0.0015	0.0103	1.6	0.90	16	6

^aActivity was determined towards (+)-camphor and analyzed by GC-MS.

Activity of the purified protein containing imidazole prior to the size exclusion chromatography could not be determined, since imidazole interferes with the used GC-MS column.

^bProtein amount as determined by the BCA assay.