Table 2.

conversion of several bicyclic ketones by crude cell extract and specific activities for pure 2,5-DKCMO

Substrate	Conversiona [%]	Specific activityb [mU/mg]
(+)-Camphor ((+)-1)	66	0.9
(-)-Camphor ((-)-1)	25	0.3
Norcamphor (13)	98	1.3
(±)-cis-bicyclo [3.2.0] hept-2-en-6-one (14)	100	1.4
(R,R)-bicyclo [2.2.1] heptane-2,5-dion (15)	94	0.06

^aConversion was determined by GC-MS analysis of biocatalysis samples with crude extract from E. coli cells overexpressing DKCMO at a substrate concentration of 1 mM after 23 h.

^bSpecific activity was calculated from conversion of substrate by pure enzyme determined by GC-MS at different points of time.